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Corning Life Sciences vertical diffusion chambers
Vertical Diffusion Chambers, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vertical+diffusion+chambers/vertical+diffusion+chambers/us11208662-1091-12-15
Average 90 stars, based on 1 article reviews
vertical diffusion chambers - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Transfection:

Article Title: Organic compositions to treat Beta-ENaC-related diseases
Article Snippet: .. To assess the ion transport phenotype of the transfected cells the Snapwell inserts are mounted in Vertical Diffusion Chambers (Costar) and are bathed continuously with gassed Ringer solution (5% CO2 in O2; pH 7.4) maintained at 37° C. containing (in mM): 120 NaCl, 25 NaHCO3, 3.3 KH2PO4, 0.8 K2HPO4, 1.2 CaCl2, 1.2 MgCl2, and 10 glucose (Osmolarity maintained between 280 and 300 mosmol/l). ..

Article Title: Organic compositions to treat Beta-ENaC-related diseases
Article Snippet: .. To assess the ion transport phenotype of the transfected cells the Snapwell inserts are mounted in Vertical Diffusion Chambers (Costar) and are bathed continuously with gassed Ringer solution (5% CO2 in O2; pH 7.4) maintained at 37° C. containing (in mM): 120 NaCl, 25 NaHCO3, 3.3 KH2PO4, 0.8 K2HPO4, 1.2 CaCl2, 1.2 MgCl2, and 10 glucose (Osmolarity maintained between 280 and 300 mosmol/l). ..

Diffusion-based Assay:

Article Title: Organic compositions to treat Beta-ENaC-related diseases
Article Snippet: .. To assess the ion transport phenotype of the transfected cells the Snapwell inserts are mounted in Vertical Diffusion Chambers (Costar) and are bathed continuously with gassed Ringer solution (5% CO2 in O2; pH 7.4) maintained at 37° C. containing (in mM): 120 NaCl, 25 NaHCO3, 3.3 KH2PO4, 0.8 K2HPO4, 1.2 CaCl2, 1.2 MgCl2, and 10 glucose (Osmolarity maintained between 280 and 300 mosmol/l). ..

Article Title: RNAi inhibition of alpha-ENaC expression
Article Snippet: .. For functional analysis in Ussings chambers, the Snapwell inserts were mounted in Vertical Diffusion Chambers (Costar) and were bathed with continuously gassed Ringer solution (5% CO2 in O2; pH 7.4) maintained at 37° C. containing: 120 mM NaCl, 25 mM NaHCO3, 3.3 mM KH2PO4, 0.8 mM K2HPO4, 1.2 mM CaCl2, 1.2 mM MgCl2, and 10 mM glucose. ..

Article Title: Organic compositions to treat Beta-ENaC-related diseases
Article Snippet: .. To assess the ion transport phenotype of the transfected cells the Snapwell inserts are mounted in Vertical Diffusion Chambers (Costar) and are bathed continuously with gassed Ringer solution (5% CO2 in O2; pH 7.4) maintained at 37° C. containing (in mM): 120 NaCl, 25 NaHCO3, 3.3 KH2PO4, 0.8 K2HPO4, 1.2 CaCl2, 1.2 MgCl2, and 10 glucose (Osmolarity maintained between 280 and 300 mosmol/l). ..

Article Title: Treating diseases mediated by blockade of the epithelial sodium channel with pyrazine-2-carboxamide derivatives
Article Snippet: .. Short Circuit Current (ISC) Measurements Snapwell inserts were mounted in Vertical Diffusion Chambers (Costar) and were bathed with continuously gassed Ringer solution (5% CO2 in O2; pH 7.4) maintained at 37° C. containing (in mM): 120 NaCl, 25 NaHCO3, 3.3 KH2PO4, 0.8 K2HPO4, 1.2 CaCl2, 1.2 MgCl2, and 10 glucose. ..

Article Title: Development of primary human nasal epithelial cell cultures for the study of cystic fibrosis pathophysiology.
Article Snippet: DEVELOPMENT OF PRIMARY HUMAN NASAL EPITHELIAL CELL CULTURES 1 FOR THE STUDY OF CYSTIC FIBROSIS PATHOPHYSIOLOGY 2 3 F de Courcey1, A Zholos2, H Atherton-Watson, MTS Williams1, P Canning1, H 4 Danahay, JS Elborn1, M Ennis1* 5 6 7 1 Centre for Infection and Immunity, Queen’s University Belfast, Health Sciences 8 Building, Lisburn Road, Belfast BT9 7BL, UK 9 10 Centre for Vision and Vascular Science, Queen’s University Belfast, Institute of Clinical 11 Science, Block A, Belfast BT12 6BA , UK and the Department of Biophysics, 12 Educational and Scientific Centre “Institute of Biology”, Taras Shevchenko Kiev 13 National University, 2 Academician Glushkov Avenue, Kiev 03187, Ukraine 14 15 Novartis Institutes for Biomedical Research, Horsham RH12 5AB, UK 16 17 Running Head: Nasal epithelial cell culture in cystic fibrosis 18 19 4 Joint senior authors 20 * Corresponding author 21 22 Correspondence details for the author are: Prof. Madeleine Ennis, Centre for Infection 23 and Immunity, Queen’s University Belfast, Health Sciences Building, Lisburn Road, 24 Belfast BT9 7AE, UK.. 25 Tel: +4428 90 972672 Fax: +44 2890972671.. E-mail: m.ennis@qub.ac.uk 26 27 Articles in PresS.

Article Title: Pyrazine derivatives as ENaC blockers
Article Snippet: .. Short Circuit Current (ISC) Measurements Snapwell inserts were mounted in Vertical Diffusion Chambers (Costar) and were bathed with continuously gassed Ringer solution (5% CO2 in O2; pH 7.4) maintained at 37° C. containing (in mM): 120 NaCl, 25 NaHCO3, 3.3 KH2PO4, 0.8 K2HPO4, 1.2 CaCl2, 1.2 MgCl2, and 10 glucose. ..

Article Title: RNAi inhibition of alpha-ENaC expression
Article Snippet: .. For functional analysis in Ussings chambers, the Snapwell inserts were mounted in Vertical Diffusion Chambers (Costar) and were bathed with continuously gassed Ringer solution (5% CO2 in O2; pH 7.4) maintained at 37° C. containing: 120 mM NaCl, 25 mM NaHCO3, 3.3 mM KH2PO4, 0.8 mM K2HPO4, 1.2 mM CaCl2), 1.2 mM MgCl2, and 10 mM glucose. ..

Article Title: RNAi inhibition of alpha-ENaC expression
Article Snippet: .. For functional analysis in Ussings chambers, the Snapwell inserts were mounted in Vertical Diffusion Chambers (Costar) and were bathed with continuously gassed Ringer solution (5% CO2 in 02; pH 7.4) maintained at 37° C. containing: 120 mM NaCl, 25 mM NaHCO3, 3.3 mM KH2PO4, 0.8 mM K2HPO4, 1.2 mM CaCl2, 1.2 mM MgCl2, and 10 mM glucose. ..

Functional Assay:

Article Title: RNAi inhibition of alpha-ENaC expression
Article Snippet: .. For functional analysis in Ussings chambers, the Snapwell inserts were mounted in Vertical Diffusion Chambers (Costar) and were bathed with continuously gassed Ringer solution (5% CO2 in O2; pH 7.4) maintained at 37° C. containing: 120 mM NaCl, 25 mM NaHCO3, 3.3 mM KH2PO4, 0.8 mM K2HPO4, 1.2 mM CaCl2, 1.2 mM MgCl2, and 10 mM glucose. ..

Article Title: RNAi inhibition of alpha-ENaC expression
Article Snippet: .. For functional analysis in Ussings chambers, the Snapwell inserts were mounted in Vertical Diffusion Chambers (Costar) and were bathed with continuously gassed Ringer solution (5% CO2 in O2; pH 7.4) maintained at 37° C. containing: 120 mM NaCl, 25 mM NaHCO3, 3.3 mM KH2PO4, 0.8 mM K2HPO4, 1.2 mM CaCl2), 1.2 mM MgCl2, and 10 mM glucose. ..

Article Title: RNAi inhibition of alpha-ENaC expression
Article Snippet: .. For functional analysis in Ussings chambers, the Snapwell inserts were mounted in Vertical Diffusion Chambers (Costar) and were bathed with continuously gassed Ringer solution (5% CO2 in 02; pH 7.4) maintained at 37° C. containing: 120 mM NaCl, 25 mM NaHCO3, 3.3 mM KH2PO4, 0.8 mM K2HPO4, 1.2 mM CaCl2, 1.2 mM MgCl2, and 10 mM glucose. ..



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Image Search Results


Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro vertical diffusion chamber (VDC) infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.

Journal: Journal of Bacteriology

Article Title: Regulatory Role of Anti-Sigma Factor RsbW in Clostridioides difficile Stress Response, Persistence, and Infection

doi: 10.1128/jb.00466-22

Figure Lengend Snippet: Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro vertical diffusion chamber (VDC) infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.

Article Snippet: The Snapwell inserts, containing polarized Caco-2, HT29, and CCD 18co myofibroblasts, were each sandwiched between two compartments of the vertical diffusion chamber (VDC) (Harvard Apparatus, UK).

Techniques: Infection, In Vitro, Diffusion-based Assay, MANN-WHITNEY

Comparison of different diffusion cell types.

Journal: Pharmaceutics

Article Title: Skin-on-a-Chip Technology for Testing Transdermal Drug Delivery—Starting Points and Recent Developments

doi: 10.3390/pharmaceutics13111852

Figure Lengend Snippet: Comparison of different diffusion cell types.

Article Snippet: Navicyte Vertical Diffusion Chamber System , Warner Instruments, Holliston, MA, USA , Static, vertical , Characterization of the intestinal, corneal, or nasal permeability , [ , , ] .

Techniques: Comparison, Diffusion-based Assay, Permeability, Dissolution, Ex Vivo

The most commonly used in vitro diffusion investigation devices.

Journal: Pharmaceutics

Article Title: The Development of an In Vitro Horizontal Diffusion Cell to Monitor Nasal Powder Penetration Inline

doi: 10.3390/pharmaceutics13060809

Figure Lengend Snippet: The most commonly used in vitro diffusion investigation devices.

Article Snippet: Navicyte Vertical Diffusion Chamber System (Harvard Apparatus, Holliston, Massachusetts, United States) , Cells, ex vivo tissue investigation Intestinal and nasal permeability , Vertical , Both phases: variable Real-time analysis is possible.

Techniques: In Vitro, Diffusion-based Assay, Ex Vivo, Permeability